1.
J Vis Exp
; (5): 215, 2007.
Artigo
em Inglês
| MEDLINE
| ID: mdl-18979015
RESUMO
Reverse genetic approaches have proven extremely useful for determining which genes underly resistance to vector pathogens in mosquitoes. This video protocol illustrates a method used by the James lab to inject dsRNA into female A. aegypti mosquitoes, which harbor the dengue virus. The technique for calibrating injection needles, manipulating the injection setup, and injecting dsRNA into the thorax is illustrated.
Assuntos
Aedes , Microinjeções/métodos , RNA de Cadeia Dupla/administração & dosagem , Aedes/virologia , Animais , Calibragem , Vírus da Dengue/isolamento & purificação , Feminino , Agulhas , Tórax
2.
J Vis Exp
; (5): 228, 2007.
Artigo
em Inglês
| MEDLINE
| ID: mdl-18979026
RESUMO
The mosquito midgut and salivary glands are key entry and exit points for pathogens such as Plasmodium parasites and Dengue viruses. This video protocol demonstrates dissection techniques for removal of the midgut and salivary glands from Aedes aegypti mosquitoes.